Effect Of Catalase On Hydrogen Peroxide

My set out is to see how the effect of catalase has on the meliorate bolt down of total heat enthalpy peroxide into pissing and oxygen. 2H202(aq) 2H20(l) + 02(g) My prediction is as you increase the engrossment of atomic number 1 peroxide the catalase go out break it down faster and therefore the fourth dimension will be quicker The individual variant is atomic number 1 peroxide I will use an separate position including the concentrations of 0. 1%, 0. 2%, 0. 5%, 1%, and 2% The dependant variable will be the time taken in seconds to measure how long the catalase takes to partitioning the hydrogen peroxide into water and oxygen. at that place were a some controlled variables. The amount of hydrogen peroxide employ was kept at a regular 10cm3. This allows for a fair running as the slaver report card would have to decease the same distance. I repeated the test an extra 3 times, allowed me to calculate a mean time. there is always a need for a repeat as it im mani fests the reliability of the experiment done. It also allows us to remove any anomalies in our results which h gives us a good jumpy estimate of the average. I carried out a suitable control experiment to prove that it was the enzyme in fact breaking down the hydrogen peroxide.In this experiment I stewed the enzyme catalase to denature it so it wouldnt be able to function properly. I and then tested this enzyme by dipping the filter report in it and then into the concentration of 2% hydrogen peroxide. As the enzyme was denatured it could non break down the hydrogen peroxide and could not get the filter paper to rise from the base of the test tube. There were many gages with this experiment. Hydrogen peroxide is an thorn that can cause irritation if unexpended on skin.Boiling water is another hazard as that can cause scold of the skin. There is also the risk of sealed crank on which anyone could cut themselves on. other danger is the Bunsen burner on which mortal could qu ite easily burn themselves. There ar procedures in place to continue these problems, wearing eye protection and wash hands after the use of hydrogen peroxide. Another prevention is keeping glass wear, boiling water and the Bunsen burner by from the edge of the table to prevent anything beingness knocked and falling off.ANALYSISMy data shows that what I predicted to be true, as you increased the concentration of hydrogen peroxide the time taken for the filter paper to reach the top of the test tube is smaller. This data is also shown in my graphs. The reliability of my results are good as they are shown to be accurate expression at other data that has been accumulated. The accuracy of my results seem high as they act to other results although improvements could be made by making sure that the level of hydrogen peroxide is accurate and more repeats are taken.My aim was to see the how catalase would breakdown hydrogen peroxide into water and oxygen and to close following my da ta and results it clearly shows that my aim was very well reached as an appropriate target. I could do further plump by changing the independent variable changing the pH. The controlled variables would be the level of hydrogen peroxide and level of water used. In this result I would notice that as the pH decreased the enzymes would denature and the breakdown of catalase would slow down.